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1996-03-09
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Document 0546
DOCN M9650546
TI Rapid molecular epidemiology of human immunodeficiency virus
transmission.
DT 9605
AU Delwart EL; Busch MP; Kalish ML; Mosley JW; Mullins JI; Aaron Diamond
AIDS Research Center, New York University School of; Medicine, New York
10016, USA.
SO AIDS Res Hum Retroviruses. 1995 Sep;11(9):1081-93. Unique Identifier :
AIDSLINE MED/96089215
AB Close sequence homology between strains of HIV-1 have been used to
corroborate cases of epidemiologically identified transmission. As an
alternative to extensive DNA sequence analysis, genetic relateness
between pairs of HIV quasispecies was estimated using the reduced
electrophoretic mobilities of HIV-1 envelope DNA heteroduplexes through
polyacrylamide gels. All six infections acquired in a dental practice in
the late 1980s and four of six infections acquired through blood product
transfusions and sexual contact in 1984-1985 could be rapidly
identified. A rising level of genetic diversity within HIV-1 subtype B
facilitated the detection of later transmission events. Transmission
linkages could be detected up to 4 years following infection. The simple
and rapid technique of DNA heteroduplex tracking can therefore assist
epidemiological investigations of HIV transmission and potentially of
other genetically variable infectious agents.
DE Base Sequence Blood Donors Blood Transfusion/ADVERSE EFFECTS Dentists
Disease Transmission, Professional-to-Patient DNA Primers/GENETICS
DNA, Viral/GENETICS Epidemiology, Molecular Genes, env Human HIV
Infections/EPIDEMIOLOGY/*TRANSMISSION/VIROLOGY
HIV-1/CLASSIFICATION/*GENETICS/ISOLATION & PURIF Molecular Sequence
Data Nucleic Acid Heteroduplexes/GENETICS Support, Non-U.S. Gov't
Support, U.S. Gov't, P.H.S. Time Factors Variation (Genetics) JOURNAL
ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).